USL League Two 2025, USA

Lansing City Football

Northern Indiana FC

Boston Bolts

New England FC

Dayton Dutch Lions

Lexington SC II

East Atlanta Dutch Lions

Dothan United SC

South Carolina United Bantams

Charlotte Independence 2

Lonestar SC

Global Football Innovation Academy

Tennessee SC

Asheville City

Virginia Marauders FC

Virginia Beach United

Birmingham Legion FC 2

Montgomery United FC

Delaware FC

Ocean City Nor'easters

Kings Hammer Columbus

West Virginia United

North Carolina FC U23

Charlotte Eagles

Seacoast United Phantoms

Vermont Green

Chicago City Dutch Lions FC

Sueno FC

Kings Hammer Sun City

Weston FC

Akron City FC

Toledo Villa FC